PEDF and 34‐mer peptide inhibit cardiac microvascular endothelial cell ferroptosis via Nrf2/HO‐1 signalling in myocardial ischemia‐reperfusion injury

Abstract Myocardial ischemia‐reperfusion injury (MIRI) represents a critical pathology in acute myocardial infarction (AMI), which is characterized by high mortality and morbidity. Cardiac microvascular dysfunction contributes to MIRI, potentially culminating in heart failure (HF). Pigment epithelium‐derived factor (PEDF), which belongs to the non‐inhibitory serpin family, exhibits several physiological effects, including anti‐angiogenesis, anti‐inflammatory and antioxidant properties. Our study aims to explore the impact of PEDF and its functional peptide 34‐mer on both cardiac microvascular perfusion in MIRI rats and human cardiac microvascular endothelial cells (HCMECs) injury under hypoxia reoxygenation (HR). It has been shown that MIRI is accompanied by ferroptosis in HCMECs. Furthermore, we investigated the effect of PEDF and its 34‐mer, particularly regarding the Nrf2/HO‐1 signalling pathway. Our results demonstrated that PEDF 34‐mer significantly ameliorated cardiac microvascular dysfunction following MIRI. Additionally, they exhibited a notable suppression of ferroptosis in HCMECs, and these effects were mediated through activation of Nrf2/HO‐1 signalling. These findings highlight the therapeutic potential of PEDF and 34‐mer in alleviating microvascular dysfunction and MIRI. By enhancing cardiac microvascular perfusion and mitigating endothelial ferroptosis, PEDF and its derivative peptide represent promising candidates for the treatment of AMI.

advancements in MIRI treatment, elucidating the precise mechanisms driving MIRI remains elusive.Hence, exploring novel therapeutic avenues is imperative.
6][7][8] Previous studies underscore PEDF's potential in mitigating vascular permeability and cardiomyocyte apoptosis in AMI rats. 9,10PEDF also could improve cardiac function in MIRI rats through inhibiting ROS generation. 11Ferroptosis, a phenomenon identified by Brent R Stockwell in 2012, 12 arises from dysregulated intracellular lipid hydroperoxide products and contributes to various physiological and pathological conditions, including ischemia-reperfusion injury. 13Notably, our recent investigation revealed PEDF's role in safeguarding endothelial barrier integrity during AMI through 67LR. 14However, the influence of PEDF and its derivative peptide on ferroptosis and vascular integrity in human cardiac microvascular endothelial cells (HCMECs) post-MIRI remains unexplored.
The transcription factor nuclear factor erythroid 2 (NF-E2)related factor 2 (Nrf2) is pivotal in maintaining cellular redox homeostasis and normal body functions, playing a crucial role in scavenging hydroxyl radicals and superoxide anions via anti-inflammatory and antioxidant mechanisms. 15,16Notably, Nrf2 activation provides cardioprotection via the coordinated up-regulation of antioxidant, anti-inflammatory, and autophagy mechanisms. 17Additionally, activation of the Nrf2/HO-1 pathway holds promise in alleviating MIRI by targeting ferroptosis. 18Thus, the aforementioned studies have confirmed the involvement of Nrf2/HO-1 in the occurrence and progression of MIRI. 19However, the involvement of Nrf2 and ferroptosis in cardiac microvascular function during MIRI remains unclear, necessitating urgent investigation.
The study unravels the PEDF and its 34-mer peptide's capacity to alleviate cardiac microvascular dysfunction and ferroptosis in MIRI rat hearts.Moreover, in vitro data demonstrate their effectiveness in inhibiting endothelial ferroptosis and modulating Nrf2/HO-1 signalling under hypoxia reoxygenation (HR).Notably, inhibiting Nrf2 diminishes PEDF's protective effect on HCAECs injury by promoting ferroptosis.Therefore, targeting the Nrf2-SLC7A11-GPX4 axis could be a promising therapeutic approach for preserving cardiac microvascular function in AMI patients.

| Animals
Sprague-Dawley (SD) male rats weighing approximately 250 ± 10 g and aged 8-10 weeks were obtained from the Experimental Animal Center of Xuzhou Medical University and accommodated in a controlled environment.All experimental procedures adhered strictly to the Guide for the Care and Use of Laboratory Animals published by the U.S. National Institutes of Health (NIH Publication, 8th Edition, 2011).The animal care and experimental protocols received approval from the Xuzhou Medical University Committee on Animal Care and complied with international ethical guidelines for animal research.

| Preparations of Lentivirus, Plasmids and Peptides
Recombinant lentivirus (PEDF-LV; Shanghai GeneChem Co. Ltd, Shanghai, China), PEDF proteins and peptides (PEDF34 and PEDF44) were prepared as previously described. 9 of the rat PEDF sequence (GenBankTM accession number NM_177927), respectively, and prepared by GL Biochem (Shanghai) Ltd., followed by high pressure liquid chromatography purification (>90% purity) and amino-terminal sequence determination.The resulting peptides were soluble in aqueous solutions.

| Rat AMI recanalization model and intramyocardial gene delivery
AMI model was established surgically by ligation of the left anterior descending (LAD) coronary artery as described previously. 8Briefly, SD rats were anesthetized with sodium pentobarbital (60 mg/kg) intraperitoneally and maintained under anaesthesia using isoflurane (1.5%-2.0%)mixed with air.After adequate anaesthesia, the animals were intubated with a 14-gauge polyethylene catheter and ventilated with room air using a small animal ventilator (Model683; Harvard Apparatus, Boston, MA).6-0 prolene monofilament polypropylene sutures were set up for AMI model building.Intramyocardial gene delivery was performed 5 days before the MI experiment in the rats.
PEDF-LV, 44-mer-LV and 34-mer-LV (2 × 10 7 TU) prepared in 20 μL enhanced infection solution (GeneChem, catalogue no.REVG0002) were delivered with a 20-μL syringe and 25-gauge needle into the myocardium along the LAD (MOI = 10).We tightened the reserved line to establish the AMI model, and released the knot to achieve coronary recanalization.We confirmed the occlusion and recanalization with ST segment elevation on the electrocardiogram.In the

| Lectin perfusion experiment
Rats were anesthetized by intraperitoneal injection of pentobarbital 90 mg/kg and 0.

| Glutathione (GSH) and malondialdehyde (MDA) assays
The relative GSH concentration in cell or tissue lysates were assessed using a Glutathione Assay Kit (G263, Dojindo).Similarly, we used a Lipid Peroxidation Assay Kit (S0131S, Beyotime) to assess the relative concentrations of malondialdehyde (MDA) in plasma or tissue lysates.We followed the manufacturers' recommended procedure for all kits in these assessments.

| Cell culture and treatment
Human cardiac microvascular endothelial cells (HCMECs; ScienCell) were used between the third and fifth passage and cultured in endothelial cell medium (ScienCell) supplemented with 5% foetal bovine serum (ScienCell), 1% endothelial cell growth supplement (ScienCell) and 1% penicillin/streptomycin solution at 37°C in a humidified atmosphere containing 5% CO 2 .We replaced the medium every 3 days.
Cells were subcultured or subjected to experimental procedures at 80%-90% confluence.To establish the hypoxia reoxygenation (HR) model, the culture medium was changed to glucose-free and serumfree medium (Gibco; Thermo Fisher Scientifc, Inc.) and placed into a tri-gas incubator (Heal Force Biomeditech Holdings, Ltd., Shanghai, China) that was purged with 94% N 2 , 5% CO 2 and 1% O 2 for 2 h.

| LDH release and cell viability assay
LDH released into the cell culture media as an indicator of lost membrane integrity and cell death.The HCMECs were seeded at a concentration of 1 × 10 4 cells/mL in 96-well plates.Following HR treatment, the cell culture media were collected, and then assessed by LDH Cytotoxicity Assay Kit (Thermo Scientific, #88953) according to the manufacturer's instructions.The cell viability was assessed using the CCK-8 kit (Dojindo, Kumamoto, Japan).Absorbance at 450 nm was measured using a microplate reader (BioTek Synergy2, VT), and the mean optical density (OD) measurements from five wells of the indicated groups were used to calculate the percentage of cell viability.

| Lipid peroxides measurement and Fe 2+ detection
To observe lipid peroxidation and Fe 2+ levels, cells were seeded on glass coverslips and treated accordingly.After treatments, cells were stained with 10 μmol/L Liperfluo (Dojindo, Japan) or 2 μmol/L FerroOrange (Dojindo, Japan) in accordance with the manufacturer's instructions.Following 30 min of incubation at 37°C in the dark, the cells were washed with HBSS and examined using a Leica SP8 con-

| Western blotting analysis
Heart tissues and cells were homogenized and incubated in lysis Thermo) and reprobed as described above.

| Statistical analysis
For all quantitative analyses, other than when specifically noted, data are expressed as mean ± standard error of the mean and repeated at least thrice in independent biological samples.Statistical significance was analysed using GraphPad Prism 9.4 software (GraphPad Software, Inc., San Diego).Two-tailed Student's t-tests were performed for two experimental groups, and one-way analysis of variance (anova) was performed for three or more experimental groups.p <0.05 was considered significant difference.

| PEDF and 34-mer inhibit myocardial ferroptosis following MIRI
The role of PEDF and 34-mer in MIRI was investigated by measuring GSH/GSSG ratio, cardiac malondialdehyde (MDA) level and Fe 2+ content in rats.In MIRI rats, GSH/GSSG ratio was significantly decreased in MIRI rats compared to sham rats, but increased when rats were pretreated with PEDF and 34-mer (Figure 2A).
Conversely, cardiac MDA levels and Fe 2+ contents were significantly higher than those in sham rats, but these levels decreased when rats were pretreated with PEDF and 34-mer (Figure 2B,C).
Furthermore, the expression of GPX4 (a selenocysteine containing protein that plays an essential role in repairing peroxidised phospholipids) and SLC7A11 (a component of the cysteine-glutamate transporter that regulates glutathione homeostasis) significantly reduced compared with the sham rats, while PEDF and 34-mer could increase the expression of GPX4 and SLC7A11 under MIRI condition (Figure 2D-F).However, 44-mer showed no significant differences compared to MIRI rats.

| PEDF and 34-mer improve cardiac microvascular perfusion through inhibiting ferroptosis
Furthermore, the EF and FS values in PEDF and 34-mer rats were superior to those in IR + Fe-citrate(III) rats (Figure 3A protective effects on microvascular perfusion through the inhibition of ferroptosis.

| PEDF and 34-mer inhibit HR-induced ferroptosis in HCMECs
We investigated the relationship between PEDF and ferroptosis in HCMECs.We selected 2-h of hypoxia and 4-h of reoxygenation as described previously.It was observed that the administration of PEDF and 34-mer led to an increase in cell viability in HCMECs (Figure 4A).PEDF and 34-mer decreased LDH release compared to HR group (Figure 4B).PEDF and 34-mer pretreatment increased GSH/GSSG ratio compared to HR group (Figure 4C).We also meas- GSH/GSSG ratio, while PEDF and 34-mer mitigated these effects (Figure 5C).Furthermore, malondialdehyde (MDA) levels and Fe 2+ contents were significantly higher in Fe-citrate(III) group than those in control group, but these levels decreased when cells were

| DISCUSS ION
Reperfusion therapy, while crucial in ischemic heart disease (IHD) treatment, paradoxically contributes to MIRI in clinical therapy. 20,21re recently, several studies indicated that limiting reperfusion injury prevents microvascular obstruction and reduces final infarct size, thereby lowering the probability of heart failure events and improving quality of life in AMI patients. 22Our study delves into the relationship between PEDF, Nrf2 and ferroptosis, investigating their potential therapeutic roles in MIRI.We found that PEDF and its 34mer peptide significantly regulated lipid peroxides and improved cardiac microvascular perfusion after MIRI.Notably, ferroptosis, a distinct form of cell death dependent on iron-induced lipid peroxidation, was identified as a contributing factor in MIRI, 23 effectively inhibited by PEDF and 34-mer through the Nrf2/HO-1 signalling pathway.These findings highlight the potential of PEDF as a therapeutic agent for reducing myocardial injury in AMI patients.
Ferroptosis is a distinctive form of non-apoptotic cell death that depends on iron and is characterized by peroxidation of phospholipids. 12[26] Ferroptosis has been found to play a role in MIRI models, and inhibiting ferroptosis has been shown to be effective in treating AMI. 27e intricate SLC7A11-GSH-GPX4 axis acts as a defence mechanism against ferroptosis, regulating cellular redox balance and mitigating lipid peroxidation. 280][31] In the study, we investigated the role of ferroptosis in MIRI rat model and HCMECs.Our findings showed that ferroptosis exacerbates cardiac microvascular perfusion and endothelial injury.The exposure of excess iron aggravates MIRI by leading cardiomyocytes to become more sensitive to oxidative stress, promoting ferroptosis and jeopardizing cardiomyocyte viability. 32Conversely, Ferrostatin-1 attenuates myocardial ischemic reperfusion-induced ferroptosis by targeting Nrf2 signalling pathway. 33,34Fer-1 is one of the most potent and widely studied inhibitors of ferroptosis.It functions primarily by scavenging lipid peroxides, thus preventing the peroxidation of polyunsaturated fatty acidcontaining phospholipids, which is a hallmark of ferroptosis.Similar to Fer-1, liproxstatin-1 is a synthetic molecule that inhibits ferroptosis by halting lipid peroxidation.Its effectiveness has been demonstrated in various models of disease, including ischemia-reperfusion (IR) injury, highlighting its potential for clinical applications.Our findings, consistent with prior research, highlight the potential of targeting ferroptosis as a therapeutic approach in treating conditions like MIRI.Our study elucidates the active involvement of PEDF and its functional peptides, particularly 34-mer, in modulating ferroptosis and MIRI.PEDF was originally identified as a secreted protein found in high levels in circulation. 35Both 34-mer and 44-mer are functional peptides of PEDF protein.While 44-mer inherits significant bioactive properties of its PEDF protein, including anti-angiogenic and neuroprotective effects, 34-mer uniquely demonstrates the enhancement of vascular integrity. 6,9,36These distinct functionalities may stem from their binding to different receptors, namely laminin receptor (LR) for 34-mer 37 and PEDF receptor (PEDFR) for 44-mer. 38e binding of PEDF to its receptor is presumably the first step in the mediation of its physiological effects. 39,40Our results demon- ing the urgency for treatments preserving microvascular integrity. 42 follows the guidelines set by the American Heart Association that treatments capable of minimizing microvascular damage should be prioritized to protect injured myocardium. 43Our previous study demonstrated that PEDF maintained the stability of endothelial adhesion junctions (AJs) to prevent the occurrence of no-reflow.
We believe that it is the cardiac microvascular dysfunction and its secondary severe interstitial edema and local swelling of the endothelium that hinder the flow of red blood cells and ultimately lead to no-reflow.Our findings emphasize the promising role of PEDF and 34-mer in preventing no-reflow by maintaining endothelial stability and inhibiting HR-induced ferroptosis in HCMECs. 44veraging the 34-mer peptide to improve cardiac microvascular PEDF overexpression plasmids, 44-LV and 34-LV were successfully constructed and then packaged in 293 T cells.The concentrated titre of virus suspension was 2 × 10 12 TU/L.Recombinant rat PEDF (GenBankTM accession number: NM_177927) was synthesized by Cusabio Biotech, Co., Ltd.(Wuhan, China).Synthetic peptides 34-mer and 44-mer were designed from amino acid positions 44-77(DPFFKAPVNKLAAAVSNFGYDLYRLRSGAVSTGN) and 78-121(ILLSPLS.VATALSALSLGAEQRTESVIHRALYYDLINNPDIHST) focal laser scanning microscope (Leica Microsystems) equipped with a Plan Apochromat 20 × 1.40 NA oil immersion objective.Slices were collected every 1.5 μm to generate z-stacks, and each image represented the maximum projection of all slices along the z-stack.Analysis of Liperfluo or Fe 2+ was performed by measuring the fluorescence intensity of at least 180 cells for each treatment condition.These cells were from at least 10 randomly selected regions of interest across three independent experiments.Fluorescence intensity was measured by Image J (National Institutes of Health), and the mean fluorescent intensity of each group was normalized to that of the control group.

3 | RE SULTS 3 . 1 |
PEDF and 34-mer improve cardiac microvascular perfusion and enhance cardiac function following MIRITo assess the potential cardioprotective effects of PEDF in rat models of MIRI, intramyocardial gene delivery was performed 5 days before the MIRI experiment.After a four-hour reperfusion, PET perfusion imaging with13 N-NH 3 was conducted to evaluate myocardial reperfusion.Compared to MIRI rats, overexpression of PEDF significantly enhanced ischemic myocardium perfusion and reduced the volume of ischemic myocardium after coronary recanalization.Similarly, endogenous 34-mer overexpression also amplified perfusion levels and decreased ischemic myocardium volume (Figure1A-C).Moreover, lectin perfusion assays indicated decreased microvascular perfusion efficiency in MIRI rats after LAD recanalization.However, PEDF and its 34-mer showed significant improvements in microvascular perfusion efficiency compared to the MIRI group, highlighting their protective effects post-MIRI (Figure1D,E).To assess the effects of PEDF and 34-mer on the severity and prognosis of MIRI, echocardiography was used to assess cardiac function.Both PEDF and 34-mer treatments notably increased EF and FS values compared to MIRI rats (Figure1F,G).However, there was no significant difference between 44-mer and MIRI rats.
ured lipid peroxidation in HCMECs via BODIPY-C11 staining.PEDF and 34-mer pretreatment significantly reduced lipid peroxidation compared to HR group in HCMECs, while 44-mer has no such effects (Figure 4D,E).PEDF and 34-mer pretreatment increased F I G U R E 3 PEDF and 34-mer improve cardiac microvascular perfusion through inhibiting ferroptosis.(A, B) Left ventricular ejection fraction (EF%) and Left ventricular fractional shortening (FS%) measured by echocardiography.(C) Representative figures of perfused microvessels labelled by lectin-FITC in the infarct area in each indicated experimental condition (bar = 50 μm).(D) Quantification of vessels with blood perfusion in infarction zone (left ventricular wall).(E) The glutathione (GSH)/glutathione disulphide (GSSG) ratio was measured.(F-H) The protein expression of SLC7A11 and GPX4 was analysed by western blotting.Values are shown as mean ± SD; **p < 0.01 vs. the sham group;***p < 0.001 vs. the sham group; ****p < 0.0001 vs. the sham group; # p < 0.05 vs. the IR group; & p < 0.05 vs. the IR + Fe-citrate(III) group; && p < 0.01 vs. the IR + Fe-citrate(III) group; &&& p < 0.001 vs. the IR + Fe-citrate(III) group; n = 7. the expression of GPX4 and SLC7A11 compared to HR group (Figure 4C,F-H).Conversely, the addition of 44-mer showed no effects.Additionally, we added Fe-citrate(III) to aggravate ferroptosis in HR model.Compared with the HR group, Fe-citrate(III) was found to increase lipid peroxidation in HCMECs.PEDF and 34-mer pretreatment decreased lipid peroxidation compared to HR + Fecitrate(III) group in HCMECs (Figure 5A,B).Fe-citrate(III) decreased

F I G U R E 4
PEDF and 34-mer inhibit HR-induced ferroptosis in human cardiac microvascular endothelial cells (HCMECs).(A) HCMECs were subjected to 2 h of hypoxia followed by 4 h of reoxygenation, and cell survival after hypoxia reoxygenation was assessed using a CCK8 assay.(B) The supernatant was collected to detect LDH release.(C) The glutathione (GSH)/glutathione disulphide (GSSG) ratio was measured.(D, E) Intracellular lipid peroxides were detected and calculated using BODIPY-C11 fluorescence.Scale bar = 100 μm.(F-H) The protein expression of SLC7A11 and GPX4 was analysed by western blotting.Values are shown as mean ± SD; **p < 0.01 vs. the control group; ***p < 0.001 vs. the control group; ****p < 0.0001 vs. the control group; # p < 0.05 vs. the HR group; ## p < 0.01 vs. the HR group; Results are representative of four independent experiments (n = 4).pretreatedwith PEDF and 34-mer (Figure5D,E).The expression of GPX4 and SLC7A11 significantly reduced after Fe-citrate(III) addition, while PEDF and 34-mer could increase the expression of GPX4 and SLC7A11 compared with that in HR + Fe-citrate(III) group (Figure5F-H).The results demonstrated that PEDF and 34-mer exhibit protective effects through inhibiting ferroptosis in vitro.F I G U R E 5 PEDF and 34-mer inhibit Fe-citrate(III)-induced ferroptosis in human cardiac microvascular endothelial cells (HCMECs) under HR condition.(A, B) Intracellular lipid peroxides were detected and calculated using BODIPY-C11 fluorescence.Scale bar = 100 μm.(C-E) The glutathione (GSH)/glutathione disulphide (GSSG) ratio and the relative values of malondialdehyde (MDA) and the intracellular Fe 2+ were measured.Values are shown as mean ± SD;***p < 0.001 vs. the control group;****p < 0.0001 vs. the control group; # p < 0.05 vs. the HR group; && p < 0.01 vs. the HR + Fe-citrate(III) group; &&& p < 0.001 vs. the HR + Fe-citrate(III) group.Results are representative of four independent experiments (n = 4).(F-H) The protein expression of SLC7A11 and GPX4 was analysed by western blotting.Values are shown as mean ± SD; **p < 0.01 vs. the control group; ****p < 0.0001 vs. the control group; & p < 0.05 vs. the HR + Fe-citrate(III) group; &&& p < 0.001 vs. the HR + Fecitrate(III) group; Results are representative of three independent experiments (n = 3).

3. 5 || 11 of 13 LU
PEDF and 34-mer inhibit HR-induced ferroptosis through Nrf2/HO-1 signalling pathway The above results indicate that PEDF and 34-mer promote cell survival and inhibit HR-induced ferroptosis in HCMECs.PEDF and 34-mer increased Nrf2 and HO-1 mRNA and protein levels in HR-induced cells, suggesting their involvement in the Nrf2/HO-1 signalling pathway (Figure 6A-E).We also found that the addition of ML385, an inhibitor of Nrf2, in HCMECs under HR and 34-mer pretreatment led to a decrease in GSH/GSSG ratio and an increase F I G U R E 6 PEDF and 34-mer inhibit HR-induced ferroptosis through Nrf2/HO-1 signalling pathway.(A-C) The protein expression of Nrf2 and HO-1 was analysed by western blotting.Values are shown as mean ± SD; ***p < 0.001 vs. the control group; ****p < 0.0001 vs. the control group; ## p < 0.01 vs. the HR group; Results are representative of three independent experiments (n = 3).(D, E) The mRNA expression of Nrf2 and HO-1 was analysed by PCR.Values are shown as mean ± SD; **p < 0.01 vs. the control group; ***p < 0.001 vs. the control group; # p < 0.05 vs. the HR group; ## p < 0.01 vs. the HR group.(F) The glutathione (GSH)/glutathione disulphide (GSSG) ratio was measured.(G, H) Intracellular lipid peroxides was detected and calculated using BODIPY-C11 fluorescence.Scale bar = o100 μm.(I-L) The protein expression f SLC7A11, HO-1 and GPX4 was analysed by western blotting.Values are shown as mean ± SD; **p < 0.01 vs. the control group; ***p < 0.001 vs. the control group; # p < 0.05 vs. the HR group; ## p < 0.01 vs. the HR group; & p < 0.05 vs. the HR + 34-mer group; && p < 0.01 vs. the HR + 34mer group; Results are representative of three independent experiments (n = 3).et al.in lipid peroxidation when compared to the HR + 34-mer group (Figure6F-H).The protein expression level of HO-1, GPX4 and SLC7A11 was also found to be upregulated in HR + 34-mer group, while ML385 can block the beneficial effects of 34-mer (Figure6I-L).The results indicate the involvement of Nrf2/HO-1 pathway in the protective effects of PEDF and 34-mer against ferroptosis.
strate that PEDF and 34-mer but not44-mer inhibit ferroptosis and improve cardiac microvascular perfusion during MIRI.Future investigations should explore LR's mediation of the beneficial effects of PEDF and 34-mer, particularly their role in inhibiting ferroptosis post-MIRI.Peptide 34-mer may be developed in the future as a potential therapeutic agent for reperfusion therapy, thereby reducing the risk of myocardial injury in clinical AMI patients.Endothelial cells play a crucial role in the pathophysiology of IR injury.During ischemia, endothelial cells experience hypoxic stress, which disrupts their barrier function, leading to increased vascular permeability and tissue edema.Upon reperfusion, the sudden influx of blood causes a massive production of reactive oxygen species (ROS) by endothelial cells, exacerbating microvascular damage through oxidative stress. 41Microvascular dysfunction during MIRI significantly contributes to the no-reflow phenomenon, highlight- perfusion post-reperfusion therapy emerges as a potential strategy for AMI treatment.At the mechanistic level, further study should be focused on 34-mer peptide and its receptor LR about its downstream cascade signals.Despite the promising results, it is important to acknowledge the limitations of our study.Our focus on a single MIRI model warrants further investigations to generalize these findings to various myocardial ischemia models.The protective mechanisms of the 34-mer and 44-mer needs further elucidated in our MIRI model.Additionally, while observing reduced ferroptosis in PEDF and 34-mer-treated rats, the involvement of LR in this process remains unexplored and warrants future investigation to comprehensively understand the underlying mechanisms.